A RadoNorm short course entitled:
CELET: Cellular effects of high and low LET ionising radiation – introduction to radiation biology
Stockholm University, Sweden
11.11.2024 – 22.11 2024
Program of CELET 2024 and practical information
Map of University campus with marked lecture rooms
Information about the Stockholm University (look at Frescati campus)
Presentations
Protocols for the exercises
Harvesting cells for analysing aberrations exercise
Tutorial for analyzing gamma-
Image galleries and tutorials
FISH images for scoring (pdf file)
FISH images -
GammaH2AX images for scoring (ZIP file)
Micronucleus images for scoring (ZIP file)
Micronucleus images -
Micronuclei in human peripheral blood lymphocytes (RENEB tutorial)
Dicentrics in human peripheral blood lymphocytes marked by AW (tutorial)
Score sheets
Chromosomal aberrations in Giemsa stained cells (paper score sheet)
Chromosomal aberrations in Giemsa stained cells (Excel score sheet)
FISH scoring sheet (Excel calculation sheet)
Micronuclei scoring sheet (Excel calculation sheet)
Instructions for group presentations on the last day of the course
Software
Please download and install on your laptops the following software needed
for analysis of foci (for PC).
ImageJ: the software is ZIPPED to a single file. Click here to start download
Once you downloaded and unpacked the file you can start the program by clicking on ImageJ.exe
IrfanView: click here to go to the website and follow the instructions
macro gammaH2AX foci for Fiji The newer Fiji ImageJ version will also give you data output if you use this macro version (macro gammaH2AX foci for Fiji, where the text line coloc is replaced with cool). However, it does not display the foci in the stacked images, i.e. using the newer ImageJ versions you cannot do the quality control of the threshold level of foci detection.
Blue macro gammaH2AX foci To be used for blue nuclei and green foci (standard image acquisition in most labs), otherwise the same as macro gammaH2AX foci.txt.
Software CABAS for estimating the dose from aberration and MN frequencies
MN calibration curve for acute gamma radiation
Dicentric calibration curve for acute gamma radiation
Useful background information
HPA report on individual radiosensitivity -
Radiation biology text book 2023
Info about radioactive decay of the measured radionuclides
Last updated: 23 11 2024
